The Effect of Intraentorhinal Injection of Selective A1 Receptor Agonist on Piriform Cortex Kindled Seizures in Rats
Trauma Monthly: 10 (2); 103-110 Article Type: Research Article
M, et al. The Effect of Intraentorhinal Injection of Selective A1 Receptor Agonist on Piriform Cortex Kindled Seizures in Rats ,
Online ahead of Print
Objevtive: In this research the role of adenosine A1 receptors of the entorhinal cortex on piriform cortex- kindled seizures was investigated. Materials and Methods: Animals were kindled by daily electrical stimulation of the piriform cortex. Then N6-Cyclohexyladenosine (CHA), an adenosine A1 receptor agonist, at concentrations of 1,10 and 100 jiM and 1,3-dimethyl-8 cyclopenthylxanthine (CPT), an adenosine A1 receptor antagonist, at concentrations of 50 and 100 jiM were injected into the entorhinal cortex. Animals were stimulated 5 mm after drug injection and seizure parameters were recorded. Also in another group of animals, CPT (50 jiM) was injected 5 mm before CHA (100 jiM). Results: CHA at a concentration of 100 jiM decreased the afterdischarge duration (ADD), stage 5 duration (S5D), and seizure duration (SD), and increased the latency to stage 4 of the seizure (54L) significantly. CHA at a concentration of 10 jiM decreased only SD and increased S4L significantly. On the other hand bilateral microinjection of CPT into the entorhinal cortex at a concentration of 100 jiM increased ADD, 55D, and SD, and reduced 54L significantly. Pretreatment of animals with CPT (50 jiM), 5 mm before CHA (100 jiM), reduced the effect of CHA on seizure parameters. Conclusion: These results suggest that adenosine A1 receptors activity in the entorhinal cortex reduces piriform cortex kindled seizures.
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