Identification and Isolation Cell Wall Proteins of Candida Albicans, Candida ?Glabrata and Hospital Specimen Comparison of Their Electrophoresis ?Patterns
Trauma Monthly: 12 (2); 117-125 Article Type: Research Article
January 1, 2007
January 1, 2007
M, et al. Identification and Isolation Cell Wall Proteins of Candida Albicans, Candida ?Glabrata and Hospital Specimen Comparison of Their Electrophoresis ?Patterns,
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Objective: Since Candida albicans is the main cause of infection within candida factors, and regarding that Candida glabrata replacement as a producing factor is the second and third within Candida factors, the aim of this research was to evaluate the protein pattern of cell wall of Candida albicans and Candida glabrata. One of the origins which can affect in causing disease is organism protein especially cell wall protein. The objective of this research is firstly to acquire Protein pattern of cell wall in approved factors of Candida albicans and Candida glabrata in a seprate manner, and secondly to obtain protein pattern of cell wall of Candida albicans gathering from clinical resources. Resulting information can present of similarities and differences between obtained patterns and standard patterns. The results of total protein patterns of cell wall can be used in comparing them with patterns of cytoplasmic proteins and the results can be used at In vitro and molecular studies. Material and Methods. Cell wall proteins were separated after lysis and collected by the 2% SDS (Sodium Dodecyl Sulfate) method. SDS-PAGE (Sodium Dodecyl Sulfate - Poly Acrylamide Gel electrophoresis( and Dice coefficient were used to evaluate the electrophoretic pattern and percent homology respectively. Cell wall proteins were obtained by the Glass Bead Friction method. Results. Results obtained from the total protein patterns of cell walls can be used for comparing with patterns cytoplasmic proteins. These results also can be useful for studying in vitro conditions and molecular studies. Conclusion. The highest level of homology in cell wall proteins was between nail and mouth, toes and arms, sample standard C.albicans and inguinal area with 95% homology and the lowest level of homology was between toes and nail with 55% homology. The highest similar molecular weight in cell wall proteins of all isolates (35 and 64Kd) in 12 specimen, (24 Kd) in 10 specimen and (50 and 26 Kd) in 9 specimen and the lowest similar molecular weight in cell wall proteins (29,42,60 Kd) was observed in 8 specimen and (29 Kd) in one specimen of C. albicans.
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