Antifungal effects of Allium cepa and some azoles in intact forms and in combinations to each other against pathogenic yeasts
Trauma Monthly: 13 (2); 103-113 Article Type: Research Article
A, Shams Ghahfarokhi
M, Hhossein Yadegari
M, Razzaghi Abyaneh
M. Antifungal effects of Allium cepa and some azoles in intact forms and in combinations to each other against pathogenic yeasts,
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Bachground and Aim: In recent years, fungal systematic infection due to pathogenic yeast have been considered as the most important causes of dead . Current antifungal therapies using routine antifungal drugs have not completely effective,. .In last decade, different formulations of combined antifungals have tested against some pathogenic fungi which shown good synergistic effects. Materials and Methods: In present work, antifungal effects of Allium cepa and some azoles e.g .fluconazole (Flu). Itraconazole (It) and ketoconazole (Kcz) were studied in intact forms and also in combinations to each other against some pathogenic yeasts including Candida albicans PTCC5057,Candida dubliniensis CD36,Cryptococcus neoformance CNE1 and Malassezia furfur MF1,invitro. The microdilution method was used for assessing antifungal susceptibility of above-mentioned compouneds in two culture media sabouraud dextrose broth (for all fungi except M.furfur) and modified Dixon broth (for only M.furfur). MIC and MFC values were calculated for each compound based on comparing colony counts of test and control samples. Findings: On the basis of obtained results, Allium cepa and azoles were inhibited the growth of all fungi tested in a dose-dependent manner. MIC ranges of Allium cepa, in combination with Flu ,It and Kcz for C.albicans (0.25-32 , 0.25-32 , 0.25-16 µg/ml) , Candida dubliniensis (0.25-32, 0.25-8, 0.125-4 µg/ml), C. neoformance (0.125-32, 0. 25-32, 0.125-16 µg/ml) and M. furfur (0.25-8, 0.125-1, 0.06-1 µg/ml) were obtained, respectively.The compounds showed higher antifungal activities in all combined formulations as compared with their alone forms(P<0.05). Results:Our results showed that Allium cepa is more effective than Flu on Candida albicans.Allium cepa and Flu have a same effect on Mallassezia furfur,but Flu and Allium cepa more effective than Allium cepa on Candida dubliniensis and Cryptococcus. neoformance .Also Allim cepa is more effective than It and Kcz on all yeasts.Allium cepa in combinations to azoles is the most effective on Malassezia furfur.
Allium cepa; Fluconazole; Itraconazole; Ketoconazole; Pathogenic yeasts; Synergistic effects; Antifungal susceptibility
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