mportance of galanin receptors type 2 in inhibitory effects of low frequency stimulations on perforant path kindling acquisition in rats
Trauma Monthly: 13 (2); 83-93 Article Type: Research Article
M, Mirnajafi Zadeh
A. mportance of galanin receptors type 2 in inhibitory effects of low frequency stimulations on perforant path kindling acquisition in rats,
Online ahead of Print
Introduction: In the present study, the impotency of galanin receptors 2 (GalR2) in the inhibitory effect of LFS on perforant path kindling acquisition was investigated. Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner (6 stimulations per day). LFS (0.1 ms pulses at 1 Hz, 600 pulses, and 50-150 µA) was applied immediately after termination each kindling stimulation. M871 (1.0 µM per site) a selective galanin receptor type 2 receptor (GalR2) antagonist, were daily microinjected into the dentate gyrus before starting the stimulation protocol and behavioral seizure stages and afterdischarge durations were recorded.. The expression of GalR2 in the dentate gyrus of different groups of animals was also compared using semi-quantitative RT-PCR. Results:Intra-dentate gyrus microinjection of M871 significantly prevents the inhibitory effect of LFS on kindling acquisition. Semi-quantitative RT-PCR showed that after kindling acquisition, the GalR2 mRNA level decreased in the dentate gyrus but application of LFS prevented this decrease. Conclusion:According to these data it may be suggested that activation of galanin receptors by endogenous galanin has an important role in mediating part of the inhibitory effects of LFS on perforant path kindled seizures. This role is mainly exerted through GalR1 during focal- and through GalR2 during generalized-kindled seizures.
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