Evaluation the Effect of Candida Albicans Infections (Candidiasis) on Tumor Mass Status, intra Tumor CD4/CD8 Ratio of the Mammary Tumor in Balb/C Mice
Trauma Monthly: 12 (1); 29-40 Article Type: Research Article
January 1, 2007
January 1, 2007
A. Evaluation the Effect of Candida Albicans Infections (Candidiasis) on Tumor Mass Status, intra Tumor CD4/CD8 Ratio of the Mammary Tumor in Balb/C Mice,
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Introduction. The aim of this study was to evaluate the effect of C. albicans infection (Candidiasis) and it's structural and secretion proteins on tumor mass status, intra-tumor CD4/CD8 ratio of the mammary tumor in Balb/c mice (in vivo) and also immunosuppressive effect of C. albicans proteins in in vitro condition. Material and Methods. C. albicans was cultured in liquid culture media and then secretary proteins were collected and purified by gel filtration chromatography. Structural proteins were obtained by sonication of the yeast and the death and survival of the mice were considered too. Structural and secretory proteins of C. albicans were evaluated by MTT assay. After that, tumor tissue was separated from breast cancer bearing mouse and then transplanted subcutaneously to syngenic Balb/c mice. Then, tumor-developed mice were located in 4 groups. In first group the mice were infected with C. albicans by IV injection. Second group received structural proteins, third group received fraction of secratory proteins and fourth group received PBS daily. Intra-tumor CD8/CD4 ratio were analyzed by flowcytometry. Results. MTT assay revealed that structural and secretory proteins of candida albicans cause suppression of proliferation responses (P<0.05). All groups of mice which received proteins and fraction of candida albicans or infected with candida albicans had significant increase in tumor volume compared with control group. Survival of the mice in all test groups decreased compared with the control group. Analysis of flowcytometry showed that structural and secretory proteins of candida albicans and infection with candida albicans cause decrease in intra-tumor CD4/CD8 ratio. Conclusion. Analysis of flow cytome try revealed that, although tumor mass increased, but the contributory cells (TCD4) could not be T helper. By these results we understood that TCD4 cells in tumor are regulatory T cells that suppress immune response and cause increase in tumor mass and progression of tumor. Structural and secratory proteins of candida albicans showed immunosuppressive effects on proliferation of lymphocytes and we can consider these proteins as a factor of increasing mass tumor and progression of malignancy in breast cancer patients.
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