Study of the effects of alcohlic extract of Crocus sativus on the acquisition and expression of morphine-induced conditioned place preference in rats
Trauma Monthly: 13 (3); 197-210 Article Type: Research Article
N, Eidi vajargah
F, et al. Study of the effects of alcohlic extract of Crocus sativus on the acquisition and expression of morphine-induced conditioned place preference in rats,
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Objective: In the present study, the effects of alcohol extract of Crocus sativus stigma on the acquisition and expression of morphine-induced conditioned place preference (CPP) in male Wistar rats (250-300 g) were investigated. Material and Methods:This experimental study was conducted on the 84 male rats that were divided into 18 groups (n=6/group). In a pilot study, different doses of morphine (0.5, 1, 2.5, 5, 7.5 and 10 mg/kg) and the extract (10, 50 and 100 mg/kg) were injected to the animals for evaluation of the drugs ability to induction of place preference. In the second phase of the experiments, the extract of the C. sativus was administered during or after induction of morphine CPP. Then, CPPs were tested in the animals. One-way Analysis of Variance (ANOVA) was proformed for statistical procedure. Administration of morphine (0.5, 1, 5, 7.5 and 10 mg/kg), indcreased the time spent in the compartment paired with morphine (i.e. conditioned place preference-CPP). The increament was significant for the dose 10 mg/kg of morphine. Administration of the plant extract (10, 50 and 100 mg/kg) did not change the time spent in the drug-paired side by the animals. Injection of the same doses of the extract before morphine (10 mg/kg) administration, caused a decrease in the time spent in drug-paired side in doses 50 and 100 mg/kg of the extract. In addition, injection of the plant extract in the test day to the animals in which received morphine (10 mg/kg) in the conditioning days decreased the expression of morphine CPP in the animals and this was statisticaly significant for doses 50 and 100 mg/kg of the extract. Results: It could be concluded that injection of the alcoholic extract of C sativus can inhibit the acquisition and expression of morphine-induced CPP in the rats. Conclusion:
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