Effect of topical application of honey on skin wound healing with full thickness in male rats
Trauma Monthly: 13 (3); 169-178 Article Type: Research Article
H, et al. Effect of topical application of honey on skin wound healing with full thickness in male rats,
Online ahead of Print
Objective:In this research the effect of topical application of commercial unboiled honey on open wound healing in male rats were studied.
Materials & Methods:50 male rats with weigh of 225±25gr randomly were chosen. Under general anesthesia and sterile condition one round wound with 2cm diameter and full thickness was made on dorsum of neck skin in rats. The day of surgery was considered as zero. Unboiled honey was applied topically on wound of experimental group every two day from time of surgery until appropriate day, control groups wounds were washed with saline. At day three, seven, fourteen and twenty eighth followed surgery rats were killed and samples from wound and adjacent skin were obtained and prepared for routine histological study and stained by H&E and vangisen. Vessels were counted on 3,7and 14th day and depth of granulation tissue was measured on 7th day. Population of fibroblasts, inflammatory cells and chrome of stained collagen were classified. Tensile strength study was done at 28 day after surgery Data were analyzed by Student-t test.
Results: Results showed significant increase in granulation tissues depth (P<0.05). and increase in maximum force for tearing healed wound (P<0.05).angiogenesis was more than control but it wasn’t significant. Density of fibroblasts, emerge of collagen and subsidence of inflammation in experimental group were higher than control Conclusion:It seems that topical application of honey accelerate wound healing in skin open wound.
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