Effect of WIN 55212-2 in Diazinon-induced toxcicity and its interaction with N-methyl-D-aspartate receptor in PC12 cell line
Trauma Monthly: 14 (4); 197-204 Article Type: Research Article
A R, Sahraei
H. Effect of WIN 55212-2 in Diazinon-induced toxcicity and its interaction with N-methyl-D-aspartate receptor in PC12 cell line,
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Aims:In this study, the protective effect of WIN 55212-2 (CB1 receptor agonist) and its interaction with N-methyl-D-aspartate receptor in diazinon-induced toxcicity in PC12 cells was evalued. Materials & Methods:In this study, PC12 maintained in DMEM+F12 culture medium supplemented with 10% fetal bovin serum. The cells were treated with diazinon (200 μM) in the presence or absence of WIN 55212-2 (0.1 μM), NMDA (100 μM), AM251 and MK801 (1 μM) in 15 minutes intervals. After 48 hours of exposure, cells viability and morphology were evaluated by MTS kit and invert light microscope, respectively. Results:Following the exposure of PC12 cells to diazinon (200 µM), a reduction in cell survival was observed (p<0.001). Treatment of the cells with WIN 55212-2 (0.1 µM) and NMDA (100 µM) prior to diazinon exposure, significantly elevated cell survival (p<0.01). Also, AM 251(1 μM) did not inhibit cell survival increase induced by WIN 55212-2 (p<0.001). However, MK801(1 μM) inhibited cell survival increase induced by NMDA (p<0.001). Conclusion. WIN 55212-2 and NMDA protect PC12 cells against diazinon-induced toxicity.
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