Effect of pre-treatment with phenylephrine on cardiac enzymes in regional ischemia/reperfusion in the isolated rat heart
Trauma Monthly: 14 (4); 189-195 Article Type: Research Article
M. Effect of pre-treatment with phenylephrine on cardiac enzymes in regional ischemia/reperfusion in the isolated rat heart,
Online ahead of Print
Aims: Although reperfusion is a valuable method for the survival of ischemic heart, it has additional deleterious effects. The aim of this study was to evaluate the preconditioning effect of phenylephrine on ischemia/reperfusion (I/R) injury and to assess the role of mitochondrial ATP-sensitive K+ channels (mitoKATP) and permeability transition pore (mPTP) in rat heart. Materials & Methods: Anesthetized rat heart was isolated and perfused with Krebs buffer in Langendorff apparatus. In control group, only the surgery procedure was performed. In second group, 30 minutes of regional ischemia followed by 60 minutes of reperfusion. In third group, phenylephrine was perfused 10 min before I/R. Forth an fifth groups were like third but respectively received 5-HD (100 µmol/l) and Atr (20 mmol/l). In sixth group, phenylephrine was injected intraperitoneally 24 hours before I/R. Seventh an eighth groups were like sixth but respectively received 5-HD and Atr. Results:Performing I/R cause the level of LDH ( lactate dehydrogenase ) and CKMB ( creatine kinase -MB) heart enzymes to increase in the coronary effluent collected in 5 and 60 minutes of reperfusion. Preconditioning with phenylephrine reduced the enzyme level and using 5-HD and Atr abolished the effect of phenylephrine. Conclusion:Phenylephrine preconditioning induces early and late cardioprotection via opening of mitoKATP channel and inhibition of mPTP in the isolated rat hearts.
Ischemia; Reperfusion; Phenylephrine; Lactate Dehydrogenase; Creatine
© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.