Melatonin dose-dependent effects on oocyte maturation capacity, in vitro fertilization and blastocyst development in mouse
Trauma Monthly: 16 (2); 67-71 Article Type: Research Article
M, Asghari Nohadani
Z. Melatonin dose-dependent effects on oocyte maturation capacity, in vitro fertilization and blastocyst development in mouse,
Online ahead of Print
Aims: Considering that m elatonin is known as an effective ROS scavenger, it may be able to improve the in-vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of melatonin on in-vitro oocyte maturation and fertilization and cleavage and blastocyst development rate in mice.
Materials and Methods: This experimental study was carried out in the cellular-molecular center of Gilan Medical University in 2010. The cumulus-oocyte complex (COC) and germinal vesicle were obtained from mice. COCs were cultured for 18 hours in maturation medium (supplemented with different doses of melatonin (100, 10, 1 m M, 100, 10 m M) and control group and their maturation rate was assessed. Then two pre-nucleus embryos were cultured in T6 medium supplemented with different doses of melatonin and embryos’ developmental rate was recorded until blastocyst stage by inverted microscope. Data were analyzed using Chi-square test by SPSS 13 software.
Results: 1, 10 and 100 m M concentrations of melatonin had positive effect on in-vitro oocyte maturation and fertilization in mice compared to the control group (P<0.01). In addition, the cleavage and blastocyst development rate increased in presence of 10and 100 nM of melatonin (P<0.01).
Conclusion: Melatonin as an effective ROS scavenger has positive influence on in-vitro oocytes maturation and embryos’ development promotion in mice.
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