The Effect of Citrullus Colocynthis Extract on Preventing/ Reducing Streptozotocin- Induced Diabetes in Rat
Trauma Monthly: 12 (1); 13-20 Article Type: Research Article
January 1, 2007
January 1, 2007
H, et al. The Effect of Citrullus Colocynthis Extract on Preventing/ Reducing Streptozotocin- Induced Diabetes in Rat,
Online ahead of Print
Introduction. The aims of this study was investigation of different parts of Citrullus Colocynthis fruit extract on Glucose concentration and antioxidant system in diabetic rat induced by Streptozotocin. Material and Methods. We investigated glycemia and antioxidant system, in six groups of rats [I, II, III, IV, V, VI] normal, diabetic control, diabetic treatment with shelf extract (200 mg/kg), diabetic treatment with shelf and core extract (200 mg/kg), prediabetic treatment with shelf extract (200 mg/kg) plus STZ injection, prediabetic treatment with shelf and core extract (200 mg/kg) plus STZ injection, respectively. Groups III and IV were treated daily with 200 mg/kg of Citrullus Colocynthis extract for 3 weeks and groups V and VI were pretreated with Citrullus Colocynthis extract for 2 weeks and then STZ was injected. Fasting blood glucose (FBS), Glutathione (GSH), superoxide dismutase (SOD) and malone dialdehyde (MDA) in spleen, liver and kidney were measured. Results. Increased glycemia in diabetic rats vs control rats (209.6 ± 18.56, 105.6 ± 18.56 mg/dl respectively) but after treatment with Citrullus Colocynthis fruit extract glucose concentration was reduced 12.5% and 22.48% respectively. GSH and SOD activities were lower in diabetic vs control rats 21 days after STZ injection but MDA was increased. Glycemia and MDA were negatively correlated with treatment and GSH concentration and SOD activity were increased significantly in all tissues. The effect of Citrullus Colocynthis fruit extract on prevention and development of diabetes was investigated by pretreatment of rats (groups Vand VI) and then STZ was injected as the same as the other groups. 25% reduction in the frequency of diabetes in pretreatment animals was shown . Conclusion. The results showed that oral Citrullus Colocynthis fruit extract is useful for prevention/reduction of development of diabetes through the lowering of oxidative stress and glycemia.
© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.