Comparison of the Virulence of Saprophytic and Pathogenic Isolates of Candida Albicans Using Murine Model of Systemic Candidiasis
Trauma Monthly: 11 (4); 331-336 Article Type: Research Article
January 1, 2007
January 1, 2007
A. Comparison of the Virulence of Saprophytic and Pathogenic Isolates of Candida Albicans Using Murine Model of Systemic Candidiasis,
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Objective. Candida albicans isolates from patients and healthy hosts have been assumed as virulent and avirulent strains respectively by some researchers studying virulence factors of the pathogenic yeast. The present study using a standard virulence determination method was performed to evaluate the assumption validity. Material and Methods. Candida albicans isolates from patients with candidiasis or normal hosts were maintained on appropriate culture media. Blastoconidia suspensions (1×10 6 cells) of each isolate inoculated to a group of BALB/c mice through their lateral vein tail. Time of mortality was recorded and the distribution of survival time was determined and analyzed by Life Table statistics method. Results. The results indicated that the studied isolates of Candida albicans were considerably different from each other in pathogenic potential as Mean Survival Time (MST) of inoculated mice varied from 5 to 26.2 days for the most virulent and the least virulent strains, respectively . However, amongst the yeasts isolated from patient and healthy hosts, both high virulent and low virulent strains were found, and there was no significant difference between the MST of the isolates from patient (13.7 ± 1/1 days) and healthy (12.45 ± 1.2 days) hosts. Conclusion. It is concluded that the host status (healthy or patient) does not predict the virulence of Candida albicans isolates, and the assumption that patient ’s isolates are high virulent may not be valid. The results also confirm that there is no way more exact than animal model to determine virulence of Candida albicans strains.
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