The Synergistic Effect of Cytokines and VitamineD3 on Differentiation of Leukemic Cells Treated with Cytotoxic Agents
Trauma Monthly: 11 (2); 135-141 Article Type: Research Article
M. The Synergistic Effect of Cytokines and VitamineD3 on Differentiation of Leukemic Cells Treated with Cytotoxic Agents,
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Aims. The Synergistic Effect of Cytokines and VitamineD3 on Differentiation of Leukemic Cells Treated with Cytotoxic Agents. Methods. The present study is based on a combined differentiation regimn consisting of differentiation agent (VD3), cytokins (INF- γ and GM-CSF) and chemotherapy (Cytosine-arabinoside and vincristin)on HL60 cells as model of acute promyelocytic leukaemia. Counting of cells morphology and peroxidase staining, NBT reduction performed to assess activity of ptoliferation and differentiation of cells after 5 days of induction. Cytofluorescent analysis performed by flow cytometry using CD45, CD14 and CDllb markers. Results. The result showed that at optimal and non toxic concentration of all druges proliferation cessed alone or in combination with VD3 .The maximal cessation of proliferation was observed by combination of VD3,cytokins and cytotoxic agant (P<0.05). All drugs except vincristine showed differetiation effect .Combination with VD3 resulted differentiation using all drugs (P<0.05).The maximal differentiation was observed in combination of VD3 ,cytokins with or without cytotoxic agents leading to granulocytic lineage. Conclusion. These data showed that combination of VD3 and cytokins provide promotion of differentiation and combination with optimal concentration of cytotoxic drugs caused maximal effect on proliferation without cessation of differentiation.
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