Effect of water extract of saffron (Crocus sativus) on chronic phase of formaline test in female mice
Trauma Monthly: 14 (1); 5-6 Article Type: Research Article
H, et al. Effect of water extract of saffron (Crocus sativus) on chronic phase of formaline test in female mice,
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Aims: In the present study, the antinocieceptive activity of saffron ( Crocus sativus) water-extract in female N-MARI mice (20-25 g) investigated.
Materials & Methods: Formalin test applied for pain assesment. 30 minutes before 2% formalin solution (2 µl) injection in posterior plantar angle, the drugs administered to the animals. Then the animals evaluated for pain assesment for 60 min in a 5 min interwalls manner.
Results: Morphine (1, 5 and 10 mg/kg) administeration induced antinocieception in both acute and chronic phases of formalin test in a dose-dependent manner. Extract (10, 50 and 100 mg/kg) also induced analgesia in chronic but not acute phase of formalin test. Naloxone (4 mg/kg) administration had no effect on the antinocieceptive activity of the extract. Dextromethorphane (20 mg/kg), on the other hand, enhances the extract action in acute phase as well as chronic phase. In addition, L-NAME (10 mg/kg) administration potentiate the extract effect. Co-administration of ineffective dose of indomethacine (2.5 mg/kg) with the extract (2.5 mg/kg) had no influence on the extract antinocieception effect.
onclusion: Water extract of C. sativus exerts its effect on the chronic phase of formalin test, possiblly via inhibition of NMDA glutamatergic as well as nitric oxide system.
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