PCR and RFLP of ureC (glmM) gene for identification and typing of Helicobacter pylori strains isolated from gastric biopsy specimens of gastric patients
Trauma Monthly: 14 (3); 143-148 Article Type: Research Article
M R, Alipour Shadbad
G A, Imani Fooladi
A. PCR and RFLP of ureC (glmM) gene for identification and typing of Helicobacter pylori strains isolated from gastric biopsy specimens of gastric patients,
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Aims: Helicobacter pylori is one of the important pathogens that infected epithelial cells of stomach. The aim of this study was to analyze and type Helicobacter pylori isolated from gastric biopsy specimens with ureC (glmM) gene. Materials & Methods: Molecular method was used in order to type Helicobacter pylori. PCR-RFLP was used to determine the genetic heterogenesity of 37 specimens of Helicobacter pylori isolated from culture. 820bp amplified fragment of ureC gene was digested with the restriction enzymes HhaI, MboI. Results: 11 different patterns with HhaI and 8 different patterns with MboI were identified. In combination, 21 different RFLP patterns were identified. Conclusion. Based on the results of this study, there is no significant relationship between gastric diseases and different patterns and there is a widespread genetic diversity between the fragments. This is promising the production of effective equipment for molecular analysis of epidemiology and analyzing ancestors and understanding the bacterial origin and its scattering in different geographic regions.
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