Simultaneous detection of common bacterial meningitis: Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumonia by multiplex PCR
Trauma Monthly: 14 (3); 119-126 Article Type: Research Article
R, Mahrabi Tavana
S M J, Karami
Z, et al. Simultaneous detection of common bacterial meningitis: Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumonia by multiplex PCR,
Online ahead of Print
Aims: Rapid treatment of bacterial meningitis is a medical emergency. Reducing mortality is depending on rapid detection. Aim of this study was to setup multiplex PCR method for detection of common bacterial meningitis.
Materials & Methods: In this resaerch, the universal and specific primers were used. Genome of 20 bacterial strains were extracted and PCR was carried out under standarded condition in two steps. The PCR products were electrophoresed on 1% agarose and compared with standard strains. Results were confirmed by using Hea III.
Results: Genome extraction of at least 10 bacteria in millilitre of fluid culture was necessary for detection. Using the universal primer of 16SrRNA made possible the amplification of 1000bp sequence. This common sequence was seen in all studied bacterial strains. By using specific primers for Nisseria meningitides, Haemophilus influenzae and Streptococcus pneumoniae a 700bp, 500bp and 300bp sequence were produced, respectively.
Conclusion: The molecular method identifies bacterial meningitis within about 3 hours and recognizes its source. Using this method in medical laboratories improves the quality and also decrease the time of detection.
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