Effect of oral morphine consumption on hippocampus development in Wistar rats embryo
Trauma Monthly: 15 (1); 11-12 Article Type: Research Article
E, Hajizadeh Moghadam
H. Effect of oral morphine consumption on hippocampus development in Wistar rats embryo,
Online ahead of Print
Aims: In the present study, the effect of oral morphine consumption on hippocampus development in Wistar rat embryos of weight range of 250-300g was investigated. Materials & Methods: 12 pregnant Wistar rats with weight range of 250-300g were fed daily with 0.05 g morphine solution in water until day 19 of pregnancy. Then, pregnant rats went under surgery and the embryo was removed with the uterus and was fixed in formalin 10% for 60 days. Then, using some stages such as processing, cutting and staining, the samples were provided and evaluated using light microscope and MOTIC software. Results: Diameter of inner, middle and an outer layer of hippocampus reduced in the experimental group comparing to control group while, the cell number increased in experimental group comparing to control group but their cell size was considerably reduced. Conclusion: Oral morphine consumption may delay hippocampus development in Wistar rat embryos, which it is seen in cell increment of this area to its different layers and layer diameter decrement of each layer.
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