Comparison of protective effects of N-acetyl-cysteine and Hexamethylenetetramine on sulfur mustard induced pathological effects in human skin fibroblast cell line HF2FF using electron microscope
Trauma Monthly: 15 (1); 1-9 Article Type: Research Article
M, Zareei Mahmuodabadi
A, Pirzad Jahromi
J. Comparison of protective effects of N-acetyl-cysteine and Hexamethylenetetramine on sulfur mustard induced pathological effects in human skin fibroblast cell line HF2FF using electron microscope,
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Aims: In this study the protective effect of hexamethylenetetramine (HMT) and N-acetyl-cysteine (NAC) were compared against sulfur mustard (HD) on human skin fibroblast cells line HF2FF. Materials & Methods: The effects of HD (180 µM) and simultaneous or pre treatment with HMT (15 µM) and NAC (0.1 mM) on cell death and cell organelles were investigated using electronic microscope. Results: The HMT and NAC prevented cell death more than 50% and about 35%, respectively. EM observation evealed that HD caused destruction of cell membrane and organelles (mitochondria, lysosome, etc.) and cell necrosis signs. HMT caused an increase in normal cells with intact lysosom es, but the reticulum endoplasmic and Golgi bodies were partially vacuolated and the Struma of some mitochondria was lost. In NAC group mitochondria were concentrated. Cytoplasm was not normal and nuclear had some signs of vacuolization, but destruction was less than control and cell organelles such as Golgi bodies and lysosomes remained intact. Conclusion: HD induces the destructive effects immediately after exposure and pretreatment is the most effective way. Application of either HMT or NAC strongly prevents the cell death and the organelles changes. The protective effect of HMT against the cell death is higher than NAC’s.
Sulfur Mustard; HF2FF Fibroblast Cell Line; Hexamethylene Tetramine; N-Acetyl-Cysteine (NAC)
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